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Sinclair College

BTN 2231 Lab for Molecular Biology Techniques

Structure and function of protein and nucleic acids, DNA replication mechanisms, DNA cloning, genetic engineering techniques, use of plasmids, nucleic acid analysis by electrophoresis, Southern hybridization, DNA amplification and sequencing, introduction to public domain DNA and protein sequence databases, use of software and internet resources for database searching.

Division: Science, Mathematics and Engineering
Department: Biotechnology
Repeatable Credit: No
Offered Online: No

Prereqs: NONE  

Outcomes

  • Perform Southern blot transfers and hybridizations by preparing DNA digests and developing DNA probes.
  • Perform various molecular biology techniques used to genetically modify a living microorganism.
  • Perform agarose gel electrophoresis, analyze results and develop conclusions.

Credit Hours: 0